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Detecting isocitrate dehydrogenase gene mutations in oligodendroglial tumors using diffusion tensor imaging metrics and their correlations with proliferation and microvascular density
Author(s) -
Xiong Ji,
Tan WenLi,
Pan JiaWei,
Wang Yin,
Yin Bo,
Zhang Jun,
Geng DaoYing
Publication year - 2016
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.24958
Subject(s) - isocitrate dehydrogenase , diffusion mri , effective diffusion coefficient , receiver operating characteristic , idh1 , fractional anisotropy , medicine , immunohistochemistry , magnetic resonance imaging , nuclear medicine , pathology , ki 67 , oligodendroglial tumor , oligodendroglioma , biology , cancer research , nuclear magnetic resonance , glioblastoma , gene , radiology , mutation , astrocytoma , physics , biochemistry , enzyme
Purpose Isocitrate dehydrogenase (IDH) mutations are frequently present in oligodendroglial tumors (OTs) and have prognostic value. We assessed whether diffusion tensor imaging (DTI) metrics could aid the noninvasive detection of IDH mutations and their correlations with tumor proliferation and microvascular density (MVD) in OTs. Materials and Methods Ninety patients with OTs who underwent conventional magnetic resonance imaging (MRI) and DTI were retrospectively reviewed (3T). IDH mutations were determined by immunohistochemical staining or direct sequencing. MVD and cell proliferation were evaluated by immunohistochemical staining with anti‐CD31 and Ki‐67, respectively. The Mann–Whitney U ‐test was applied to each of the imaging parameters. Spearman correlation analysis and receiver operating characteristic curve analysis were performed. Results The maximal fractional anisotropy (FA), ratio of maximal FA (rmFA), minimal ADC, and ratio of minimal (rmADC) values were demonstrated to be significantly different between the OTs with IDH1/2 mutations and those without mutations ( P < 0.05). The areas under the curve (AUCs) for the maximal FA, rmFA, minimal ADC, and rmADC were 0.79, 0.82, 0.77, and 0.80, respectively. A combination rmFA and rmADC for the diagnosis of IDH1/2 mutations led to sensitivity, specificity, and AUC of 91.5%, 76.5%, and 0.86, respectively. The Ki‐67 and MVD levels in the IDH‐mutated samples were lower than those in the IDH wildtype cases ( P < 0.05). Conclusion DTI metrics may provide a noninvasive method for assessing the IDH statuses of OTs. Significantly higher minimal ADC and lower maximal FA in OTs with IDH mutations may suggest that IDH mutations lead to proliferation inhibition and an angiogenesis decrease. J. MAGN. RESON. IMAGING 2016;43:45–54.