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Hyperpolarized choline as an MR imaging molecular probe: Feasibility of in vivo imaging in a rat model
Author(s) -
FriesenWaldner Lanette J.,
Wade Trevor P.,
Thind Kundan,
Chen Albert P.,
Gomori J. Moshe,
Sosna Jacob,
McKenzie Charles A.,
KatzBrull Rachel
Publication year - 2015
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.24659
Subject(s) - choline , magnetic resonance imaging , nuclear medicine , in vivo , hyperpolarization (physics) , flip angle , chemistry , gradient echo , molecular imaging , medicine , nuclear magnetic resonance , radiology , nuclear magnetic resonance spectroscopy , biology , physics , microbiology and biotechnology
Purpose To assess the feasibility of choline MRI using a new choline molecular probe for dynamic nuclear polarization (DNP) hyperpolarized MRI. Materials and Methods Male Sprague‐Dawley rats with an average weight of 400 ± 20 g (n = 5), were anesthetized and injection tubing was placed in the tail vein. [1,1,2,2‐D 4 , 1‐ 13 C]choline chloride (CMP1) was hyperpolarized by DNP and injected into rats at doses ranging from 12.6 to 50.0 mg/kg. Coronal projection 13 C imaging was performed on a 3 Tesla clinical MRI scanner (bore size 60 cm) using a variable flip angle gradient echo sequence. Images were acquired 15 to 45 s after the start of bolus injection. Signal intensities in regions of interest were determined at each time point and compared. Results 13 C MRI images of hyperpolarized CMP1 at a 50 mg/kg dose showed time‐dependent organ distribution patterns. At 15 s, high intensities were observed in the inferior vena cava, heart, aorta, and kidneys. At 30 s, most of the signal intensity was localized to the kidneys. These distribution patterns were reproduced using 12.6 and 25 mg/kg doses. At 45 s, only signal in the kidneys was detected. Conclusion Hyperpolarized choline imaging with MRI is feasible using a stable‐isotope labeled choline analog (CMP1). Nonradioactive imaging of choline accumulation may provide a new investigatory dimension for kidney physiology. J. Magn. Reson. Imaging 2015;41:917–923 . © 2014 Wiley Periodicals, Inc .