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Comparison of retinal and cerebral blood flow between continuous arterial spin labeling MRI and fluorescent microsphere techniques
Author(s) -
Shih YenYu I.,
Garza Bryan H.,
Huang Shiliang,
Li Guang,
Wang Lin,
Duong Timothy Q.
Publication year - 2014
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.24407
Subject(s) - microsphere , arterial spin labeling , fluorescence , cerebral blood flow , retinal , blood flow , biomedical engineering , medicine , nuclear magnetic resonance , radiology , cardiology , ophthalmology , physics , optics , chemical engineering , engineering
Purpose To compare basal retinal and cerebral blood flow (BF) values using continuous arterial spin labeling (CASL) MRI and fluorescent microspheres. Materials and Methods A total of 41 animals were used. BF was measured using an established microsphere technique (a mixture of 2.5 million 8 μm green and 0.5 million 10 μm blue fluorescent microspheres) and CASL MRI blood flow measurement in the rat retina and brain at 7 Tesla (T) and 11.7T, respectively. Results Retinal BF by MRI was 1.18 ± 0.57 mL/g/min and choroidal BF was 8.14 ± 1.8 mL/g/min (n = 6). Microsphere retinal BF was 9.12 ± 2.8 μL/min per tissue and choroidal BF was 73.38 ± 44 μL/min per tissue (n = 18), corresponding to a retinal BF value of 1.22 ± 0.36 mL/g/min by means of a wet weight conversion. The wet‐weight of the choroid could not be determined. To corroborate our findings, cerebral BF (CBF) by MRI was also analyzed. In the cerebral cortices, CBF was 0.91 ± 0.29 mL/g/min (n = 14) by CASL MRI and 1.09 ± 0.37 mL/g/min (n = 6) by microspheres. There were no significant differences found between MRI and microsphere blood flow in the retina and brain. Conclusion BF values in the rat retina and cerebral cortex by MRI are in agreement with those obtained by the microsphere technique. J. Magn. Reson. Imaging 2014;40:609–615 . © 2013 Wiley Periodicals, Inc .