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In vivo identification of sentinel lymph nodes using MRI and size‐controlled and monodispersed magnetite nanoparticles
Author(s) -
Iida Shuji,
Imai Kensuke,
Matsuda Sachiko,
Itano Osamu,
Hatakeyama Mamoru,
Sakamoto Satoshi,
Kokuryo Daisuke,
Okabayashi Koji,
Endo Takashi,
Ishii Yoshiyuki,
Hasegawa Hirotoshi,
Aoki Ichio,
Handa Hiroshi,
Kitagawa Yuko
Publication year - 2013
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.24108
Subject(s) - magnetite nanoparticles , lymph , in vivo , magnetic resonance imaging , sentinel lymph node , lymph node , magnetic nanoparticles , magnetite , biomedical engineering , materials science , nanoparticle , chemistry , nuclear magnetic resonance , pathology , nanotechnology , medicine , radiology , physics , biology , cancer , microbiology and biotechnology , metallurgy , breast cancer
Purpose To develop a sentinel lymph node (SN) identification method using accurately synthesized magnetic nanoparticles (MNPs), as an enhanced specific SN tracer in combination with magnetic resonance imaging (MRI) in intact rodent and SN metastasis models. Materials and Methods Three sizes of MNPs were originally synthesized. We developed an experimental rat SN model, with brachial lymph nodes (Br) as the SN and the axillary lymph node (Ax) as the second lymph node, and injection of MNPs via the front paw. SN detectability was evaluated in vivo using T 1 ‐weighted MR images after injection of the synthesized MNPs, and the amount of iron in the Br and in the Ax was assessed using inductively coupled plasma optical emission spectrometry. Results The highest ratios of the amount of iron in the Br versus the Ax were 3.1 and 3.3, using 20‐nm MNPs after 2‐ and 24‐hour injections. The appropriate dose and particle diameter for MRI detection was optimized, and the SN was optimally distinguished in the normal and metastatic rat models using MRI after a 0.4 mg/kg 20‐nm MNP injection. Conclusion We developed and optimized a useful SN identification method using MRI in rodent models. J. Magn. Reson. Imaging 2013;38:1346–1355. © 2013 Wiley Periodicals, Inc.