Influence of white matter fiber orientation on R2* revealed by MRI segmentation

Purpose: To investigate white matter heterogeneity using a multichannel segmentation of a large sample of structural and diffusion magnetic resonance imaging (MRI) data. Materials and Methods: A sample of 50 subjects was segmented using channels comprising exclusively structural (longitudinal and transverse relaxation times T1 and T2 and transverse relaxation rate R2*) and diffusion‐based MRI indices (mean diffusivity and fractional anisotropy). These data were analyzed using a data driven approach in which no prior information was used. Results: The analysis revealed the splitting of white matter into two subclasses in which the longitudinal fasciculi were distinguished from inferior/superior ones. The distribution of the adopted indices in the obtained clusters showed that R2* was mainly responsible for this splitting. Conclusion: This result supports the observation, previously hypothesized in the literature, that R2* is influenced by the fiber orientation. J. Magn. Reson. Imaging 2013;37:85–91. © 2012 Wiley Periodicals, Inc.