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Assessment of liver function in thioacetamide‐induced rat acute liver injury using an empirical mathematical model and dynamic contrast‐enhanced MRI with Gd‐EOB‐DTPA
Author(s) -
Saito Shigeyoshi,
Moriyama Yuki,
Kobayashi Shuichiro,
Ogihara Ryota,
Koto Daichi,
Kitamura Akihiro,
Matsushita Taro,
Nishiura Motoko,
Murase Kenya
Publication year - 2012
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.23726
Subject(s) - thioacetamide , saline , magnetic resonance imaging , nuclear medicine , medicine , washout , alanine aminotransferase , gadolinium , liver injury , chemistry , liver function , radiology , organic chemistry
Purpose: To evaluate thioacetamide (TAA)‐induced acute liver injury in rats using an empirical mathematical model (EMM) and dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) with gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd‐EOB‐DTPA). Materials and Methods: Eighteen rats were divided into three groups (normal control [ n = 6], TAA [140] [ n = 6], and TAA [280] groups [ n = 6]). The rats of the TAA (140) and TAA (280) groups were intravenously injected with 140 and 280 mg/kg body weight (BW) of TAA, respectively, while those of the normal control group were intravenously injected with the same volume of saline. DCE‐MRI studies were performed using Gd‐EOB‐DTPA (0.025 mmol Gd/kg; 0.1 mL/kg BW) as the contrast agent 48 hours after TAA or saline injection. After the DCE‐MRI study, blood was sampled and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured. We calculated the rate of contrast uptake (α), the rate of contrast washout (β), the elimination half‐life of relative enhancement ( RE ) ( T 1/2 ), the maximum RE ( RE max ), and the time to ( RE max ) ( T max ) from time‐signal intensity curves using EMM. Results: The RE max values in the TAA (140) groups and TAA (280) groups were significantly smaller than that in the normal control group. The T max value in the TAA (280) group was significantly greater than that in the normal control group. The β value in the TAA (280) group was significantly smaller than those in the normal control and TAA (140) groups, whereas there were no significant differences in β among groups. The T 1/2 value in the TAA (280) group was significantly greater than those in the normal control and TAA (140) groups. The RE max , T max , β, and T 1/2 values significantly correlated with AST and ALT. Conclusion: The EMM is useful for evaluating TAA‐induced acute liver injury using DCE‐MRI with Gd‐EOB‐DTPA. J. Magn. Reson. Imaging 2012; 36:1483–1489. © 2012 Wiley Periodicals, Inc.