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Ultrahigh‐field DCE‐MRI of angiogenesis in a novel angiogenesis mouse model
Author(s) -
Wittenborn Thomas,
Nielsen Thomas,
Nygaard Jens V.,
Larsen Esben K.U.,
Thim Troels,
Rydtoft Louise M.,
VorupJensen Thomas,
Kjems Jørgen,
Nielsen Niels Chr.,
Horsman Michael R.,
Falk Erling
Publication year - 2012
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.22855
Subject(s) - angiogenesis , magnetic resonance imaging , in vivo , scaffold , medicine , neovascularization , pathology , biomedical engineering , radiology , cancer research , biology , microbiology and biotechnology
Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE‐MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE‐MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis‐related drugs and contrast agents. J. Magn. Reson. Imaging 2012;35:703‐710. © 2011 Wiley Periodicals, Inc.