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The presence of two local myocardial sheet populations confirmed by diffusion tensor MRI and histological validation
Author(s) -
Kung Geoffrey L.,
Nguyen Tom C.,
Itoh Aki,
Skare Stefan,
Ingels Neil B.,
Miller D. Craig,
Ennis Daniel B.
Publication year - 2011
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.22725
Subject(s) - diffusion mri , histology , population , observable , diffusion , artifact (error) , anatomy , medicine , physics , pathology , magnetic resonance imaging , biology , radiology , environmental health , quantum mechanics , neuroscience , thermodynamics
Purpose: To establish the correspondence between the two histologically observable and diffusion tensor MRI (DTMRI) measurements of myolaminae orientation for the first time and show that single myolaminar orientations observed in local histology may result from histological artifact. Materials and Methods: DTMRI was performed on six sheep left ventricles (LV), then corresponding direct histological transmural measurements were made within the anterobasal and lateral‐equatorial LV. Secondary and tertiary eigenvectors of the diffusion tensor were compared with each of the two locally observable sheet orientations from histology. Diffusion tensor invariants were calculated to compare differences in microstructural diffusive properties between histological locations with one observable sheet population and two observable sheet populations. Results: Mean difference ± 1SD between DTMRI and histology measured sheet angles was 8° ± 27°. Diffusion tensor invariants showed no significant differences between histological locations with one observable sheet population and locations with two observable sheet populations. Conclusion: DTMRI measurements of myolaminae orientations derived from the secondary and tertiary eigenvectors correspond to each of the two local myolaminae orientations observed in histology. Two local sheet populations may exist throughout LV myocardium, and one local sheet population observed in histology may be a result of preparation artifact. J. Magn. Reson. Imaging 2011. © 2011 Wiley Periodicals, Inc.