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In vivo single scan detection of both iron‐labeled cells and breast cancer metastases in the mouse brain using balanced steady‐state free precession imaging at 1.5 T
Author(s) -
Ribot Emeline J.,
MartinezSantiesteban Francisco M.,
Simedrea Carmen,
Steeg Patricia S.,
Chambers Ann F.,
Rutt Brian K.,
Foster Paula J.
Publication year - 2011
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.22593
Subject(s) - steady state free precession imaging , flip angle , echo time , gradient echo , nuclear magnetic resonance , nuclear medicine , brain tumor , in vivo , magnetic resonance imaging , glioma , medicine , physics , pathology , radiology , cancer research , biology , microbiology and biotechnology
Purpose: To simultaneously detect iron‐labeled cancer cells and brain tumors in vivo in one scan, the balanced steady‐state free precession (b‐SSFP) imaging sequence was optimized at 1.5 T on mice developing brain metastases subsequent to the injection of micron‐sized iron oxide particle‐labeled human breast cancer cells. Materials and Methods: b‐SSFP sequence parameters (repetition time, flip angle, and receiver bandwidth) were varied and the signal‐to‐noise ratio, contrast between the brain and tumors, and the number of detected iron‐labeled cells were evaluated. Results: Optimal b‐SSFP images were acquired with a 26 msec repetition time, 35° flip angle, and bandwidth of ±21 kHz. b‐SSFP images were compared with T 2 ‐weighted 2D fast spin echo (FSE) and 3D spoiled gradient recalled echo (SPGR) images. The mean tumor‐brain contrast‐to‐noise ratio and the ability to detect iron‐labeled cells were the highest in the b‐SSFP images. Conclusion: A single b‐SSFP scan can be used to visualize both iron‐labeled cells and brain metastases. J. Magn. Reson. Imaging 2011;. © 2011 Wiley‐Liss, Inc.