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Feasibility and precision of cerebral blood flow and cerebrovascular reactivity MRI measurements using a computer‐controlled gas delivery system in an anesthetised juvenile animal model
Author(s) -
Winter Jeff D.,
Fierstra Jorn,
Dorner Stephanie,
Fisher Joseph A.,
Lawrence Keith S.,
Kassner Andrea
Publication year - 2010
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.22230
Subject(s) - repeatability , cerebral blood flow , medicine , blood flow , coefficient of variation , nuclear medicine , magnetic resonance imaging , breathing , ventilation (architecture) , repeated measures design , anesthesia , biomedical engineering , cardiology , radiology , chemistry , mathematics , mechanical engineering , statistics , chromatography , engineering
Purpose: To demonstrate the feasibility and repeatability of cerebrovascular reactivity (CVR) imaging using a controlled CO 2 challenge in mechanically ventilated juvenile pigs. Materials and Methods: Precise end‐tidal partial pressure CO 2 (P ETCO 2 ) control was achieved via a computer‐controlled model‐driven prospective end‐tidal targeting (MPET) system integrated with mechanical ventilation using a custom‐built secondary breathing circuit. Test‐retest blood‐oxygen level dependent (BOLD) CVR images were collected in nine juvenile pigs by quantifying the BOLD response to iso‐oxic square‐wave P ET CO 2 changes. For comparison, test‐retest baseline arterial spin labeling (ASL) cerebral blood flow (CBF) images were collected. Repeatability was quantified using the intra‐class correlation coefficient (ICC) and coefficient of variation (CV). Results: The repeatability of the PETCO 2 (CV < 2%) step changes resulted in BOLD CVR ICC > 0.94 and CV < 6% for cortical brain regions, which was similar to ASL CBF repeatability (ICC > 0.96 and CV < 4%). Conclusion: This study demonstrates the feasibility and precision of CVR imaging with an MPET CO 2 challenge in mechanically ventilated subjects using an animal model. Translation of this method into clinical imaging protocols may enable CVR imaging in young children with cerebrovascular disease who require general anesthesia. J. Magn. Reson. Imaging 2010;32:1068–1075. © 2010 Wiley‐Liss, Inc.