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Multiparametric assessment of the anti‐glioma properties of OKN007 by magnetic resonance imaging
Author(s) -
Garteiser Philippe,
Doblas Sabrina,
Watanabe Yasuko,
Saunders Debbie,
Hoyle Jessica,
Lerner Megan,
He Ting,
Floyd Robert A.,
Towner Rheal A.
Publication year - 2010
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.22106
Subject(s) - glioma , perfusion , magnetic resonance imaging , medicine , nuclear medicine , pathology , angiogenesis , histology , perfusion scanning , radiology , cancer research
Purpose: To demonstrate that OKN007, a disulfonyl derivative of phenyl‐ tert ‐butyl nitrone (PBN), has anti‐glioma activity in the clinically relevant C6 rat glioma model using multi‐parametric magnetic resonance imaging. Materials and Methods: Twenty‐one rats were intracerebrally implanted with C6 cells and administered OKN007 or kept as controls. Animals were monitored with MRI at 7 Tesla (T), using morphologic, diffusion‐weighted and perfusion imaging, followed by histology and Western blots of angiogenesis and inflammatory markers. Results: OKN007 was found to decrease tumor volumes and increase survival. The glioma tissues of OKN007‐treated rats were found to have longitudinal apparent diffusion coefficients (ADC z ) of 0.76 ± 0.06 × 10 −3 mm 2 /s, similar to the contralateral tissue and significantly smaller than untreated gliomas (0.97 ± 0.13 × 10 −3 mm 2 /s). They had higher perfusion rates (66 ± 4 mL/100 g·min) than untreated gliomas (26 ± 7 mL/100 g·min). All examined molecular markers were decreased in OKN007‐treated rat gliomas, compared with elevated levels in untreated rats. Conclusion: MRI assessment was successfully used to monitor a decrease in tumor growth, and corresponding alterations in ADC and perfusion rates in rat C6 gliomas treated with the anti‐glioma agent, OKN007. J. Magn. Reson. Imaging 2010;31:796–806. ©2010 Wiley‐Liss, Inc.