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Noninvasive quantification of hepatic steatosis inrats using 3.0 T 1 H‐magnetic resonance spectroscopy
Author(s) -
Marsman H.A.,
van Werven J.R.,
Nederveen A.J.,
ten Kate F.J.,
Heger M.,
Stoker J.,
van Gulik T.M.
Publication year - 2010
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.22064
Subject(s) - steatosis , medicine , chemistry , fatty liver , area under the curve , magnetic resonance imaging , endocrinology , radiology , disease
Purpose: To assess the accuracy of noninvasive 3.0 T 1 H‐magnetic resonance spectroscopy ( 1 H‐MRS) in an experimental steatosis model for the discrimination of clinically relevant macrovesicular steatosis degrees and to evaluate three different 1 H‐MR spectrum‐based fat quantification methods. Materials and Methods: Steatosis was induced in rats by a methionine/choline‐deficient diet for 0–5 weeks. 1 H‐MRS measurements of hepatic fat content were compared with histopathological and biochemical steatosis degree. In 1 H‐MR spectra, areas under the curve (AUC) of fat (1.3 ppm), water (4.7 ppm), total fat (0.5–5.3 ppm), and total spectrum peaks (0.5–5.3 ppm) were determined and hepatic fat content calculated as follows: [AUC total fat peaks /AUC total peaks ], [AUC fat /AUC fat + (AUC water /0.7)], and [AUC fat /AUC water ]. Results: A significant correlation was found between 1 H‐MRS and macrovesicular steatosis ( r = 0.932, P < 0.0001) and between 1 H‐MRS and total fatty acids ( r = 0.935, P < 0.0001). 1 H‐MRS accurately distinguished mild from moderate and moderate from severe steatosis. Calculations using [AUC fat /AUC water ] ratio in severe steatotic livers resulted in higher hepatic fat percentages as compared to the other methods due to a decrease in hepatic water content. Conclusion: 1 H‐MRS quantification of hepatic fat content showed high correlations with histological and biochemical steatosis determination in an experimental steatosis rat model and accurately discriminated between clinically relevant steatosis degrees. These results encourage further application of 1 H‐MRS in patients for accurate steatosis assessment. J. Magn. Reson. Imaging 2010;32:148–154. © 2010 Wiley‐Liss, Inc.