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Investigation using an HER‐2/neu transgenic mouse model of a newly developed MR contrast agent with the effect of an antitumor drug
Author(s) -
Sonoda Akinaga,
Nitta Norihisa,
Ohta Shinich,
NittaSeko Ayumi,
Murata Satoshi,
Jo Junichiro,
Tabata Yasuhiko,
Takahashi Masashi,
Tani Toru,
Murata Kiyoshi
Publication year - 2009
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21911
Subject(s) - gadolinium , gelatin , in vitro , chemistry , diethylenetriaminepentaacetic acid , magnetic resonance imaging , conjugate , medicine , toxicity , cisplatin , mri contrast agent , nuclear medicine , chelation , chemotherapy , biochemistry , radiology , mathematical analysis , mathematics , organic chemistry
Purpose To assess whether Gd‐DTPA‐Gel‐Cis, a conjugate of gadolinium (Gd), cis diamminedichloroplatinum (Cis), diethylenetriaminepentaacetic acid (DTPA)‐dianhydride, and bovine gelatin (Gel) can be used as an intravascular contrast agent at MRI and as an antitumor cell proliferation agent in vitro. Materials and Methods We injected Gd‐DTPA‐Gel‐Cis (200 mg/mL) into the caudal vein of female HER‐2/neu transgenic mice with spontaneous mammary tumors. The tumor signal intensity was measured with a 0.3 Tesla MRI scanner. HER‐2/neu‐expressing NT cells were treated with Gd‐DTPA‐Gel‐Cis (5 μM cisplatin, 200 mg/mL Gel), Cis alone (5 μM cisplatin), or Gel alone (200 mg/mL gelatin). Differences of P < 0.05 were considered to be statistically significant. Results On T1‐weighted MRI scans of mice injected with Gd‐DTPA‐Gel‐Cis we observed a 23% increase in signal intensity. The survival rates of cells exposed to Gd‐DTPA‐Gel‐Cis or Cis were 70.9% and 58.3%, respectively, of the survival rates observed after treatment with Gel alone. Gd‐DTPA‐Gel‐Cis showed significant toxicity ( P < 0.05). Conclusion Gd‐DTPA‐Gel‐Cis shows promise for use as an MRI contrast medium and as an antitumor agent. J. Magn. Reson. Imaging 2009;30:907–910. © 2009 Wiley‐Liss, Inc.