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Gadolinium‐containing magnetic resonance image contrast agent promotes fibrocyte differentiation
Author(s) -
Vakil Varsha,
Sung Joanna J.,
Piecychna Marta,
Crawford Jeffrey R.,
Kuo Phillip,
AbuAlfa Ali K.,
Cowper Shawn E.,
Bucala Richard,
Gomer Richard H.
Publication year - 2009
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21800
Subject(s) - fibrocyte , nephrogenic systemic fibrosis , magnetic resonance imaging , gadolinium , fibrosis , peripheral blood mononuclear cell , pathology , cellular differentiation , medicine , chemistry , in vitro , radiology , biochemistry , organic chemistry , gene
Gadolinium‐containing magnetic resonance imaging (MRI) contrast agents such as Omniscan are associated with nephrogenic systemic fibrosis (NSF). To determine if Omniscan can affect the differentiation of monocytes into fibroblast‐like cells called fibrocytes that are found in the fibrotic lesions of NSF, peripheral blood mononuclear cells (PBMCs) from NSF patients, hemodialysis patients without NSF, and healthy, renally sufficient controls were exposed to Omniscan in a standardized in vitro fibrocyte differentiation protocol. When added to PBMCs, the gadolinium‐containing MRI contrast agent Omniscan generally had little effect on fibrocyte differentiation. However, 10 −8 to 10 −3 mg/mL Omniscan reduced the ability of the fibrocyte differentiation inhibitor serum amyloid P (SAP) to decrease fibrocyte differentiation in PBMCs from 15 of 17 healthy controls and one of three NSF patients. Omniscan reduced the ability of SAP to decrease fibrocyte differentiation from purified monocytes, indicating that the Omniscan effect does not require the presence of other cells (such as T cells) in the PBMCs. Omniscan also reduced the ability of a different fibrocyte differentiation inhibitor, interleukin‐12, to decrease fibrocyte differentiation. These data suggest that Omniscan interferes with the regulatory action of signals that inhibit the differentiation of monocytes to fibrocytes. J. Magn. Reson. Imaging 2009;30:1284–1288. © 2009 Wiley‐Liss, Inc.