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Noninvasive in vivo electron paramagnetic resonance study to estimate pulmonary reducing ability in mice exposed to NiO or C60 nanoparticles
Author(s) -
Yokoyama Hidekatsu,
Ono Taizo,
Morimoto Yasuo,
Myojo Toshihiko,
Tanaka Isamu,
Shimada Manabu,
Wang WeiNing,
Endoh Shigehisa,
Uchida Kunio
Publication year - 2009
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21786
Subject(s) - electron paramagnetic resonance , in vivo , nanoparticle , materials science , non blocking i/o , magnetic resonance imaging , nanomaterials , nuclear magnetic resonance , biomedical engineering , nanotechnology , chemistry , medicine , radiology , physics , biology , biochemistry , microbiology and biotechnology , catalysis
Purpose To develop new methods that can estimate the influences of manufactured nanomaterials on biological systems, the in vivo pulmonary reducing ability of mice that had received inhalation exposures to NiO or C60 nanoparticles was investigated using a 700 MHz electron paramagnetic resonance (EPR) spectrometer. Materials and Methods NiO or C60 suspensions were atomized and mice in exposure chambers inhaled the resulting aerosol particles for 3 hours. The exposure conditions, number‐based geometric average diameters, and the average number concentration were precisely controlled at almost the same levels for both NiO and C60 nanoparticles. Two days or 2 weeks after exposure, an EPR study was conducted noninvasively. Temporal changes in EPR signal intensity at the target area (ie, lung field) were obtained by the region‐selected intensity determination (RSID) method. Results NiO nanoparticles significantly suppressed pulmonary reducing ability 2 days and 2 weeks after exposure, but C60 nanoparticles had no such effect. Conclusion This is the first in vivo estimation of the reducing ability in experimental animals exposed to manufactured nanoparticles. J. Magn. Reson. Imaging 2009. © 2009 Wiley‐Liss, Inc.