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Diffusion‐weighted MRI of hepatic tumor in rats: Comparison between in vivo and postmortem imaging acquisitions
Author(s) -
Sun Xihe,
Wang Huaijun,
Chen Feng,
De Keyzer Frederik,
Yu Jie,
Jiang Yansheng,
Feng Yuanbo,
Li Junjie,
Marchal Guy,
Ni Yicheng
Publication year - 2009
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21675
Subject(s) - in vivo , medicine , necrosis , effective diffusion coefficient , diffusion mri , nuclear medicine , perfusion , magnetic resonance imaging , ex vivo , liver tumor , pathology , radiology , hepatocellular carcinoma , microbiology and biotechnology , biology
Purpose To determine the feasibility of in vivo diffusion‐weighted imaging (DWI) to distinguish between normal liver, viable tumor and necrosis compared to postmortem DWI in a rat model with vascular‐targeting treatment. Materials and Methods Fifteen rats with liver implantation of 30 rhabdomyosarcomas were treated with combretastatin A‐4‐phosphate (CA4P) at 10 mg/kg. Two days after treatment, T2‐weighted imaging, precontrast T1‐weighted imaging, postcontrast T1‐weighted imaging, and DWI were performed in vivo and postmortem with a 1.5T scanner. Apparent diffusion coefficients (ADCs) calculated from DWIs with b values of 0, 50, and 100 seconds/mm 2 (ADC low ), 500, 750, and 1000 seconds/mm 2 (ADC high ), 0, 500, and 1000 seconds/mm 2 (ADC 3b ), and 0–1000 seconds/mm 2 (ADC 10b ) for tumor, liver, therapeutic necrosis, and phantoms were compared and validated with ex vivo microangiographic and histopathologic findings. Results Except ADC low between tumor and necrosis, in vivo ADCs successfully differentiated liver, viable tumor, and necrosis (P < 0.05). Compared to in vivo outcomes, postmortem ADCs significantly dropped in tumor and liver ( P < 0.05) except ADC high of tumor, but not in necrosis and phantoms. Compared to ADC low , ADC high was less affected by vital status. Conclusion Advantageous over postmortem DWI, in vivo DWI provides a noninvasive easy‐performing tool for distinguishing between liver, viable tumor, and necrosis. ADC low and ADC high better reflect tissue perfusion and water diffusion, respectively. J. Magn. Reson. Imaging 2009;29:621–628. © 2009 Wiley‐Liss, Inc.