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USPIO (Ferumoxtran‐10)‐enhanced MRI to visualize reticuloendothelial system cells in neonatal rats: Feasibility and biodistribution study
Author(s) -
Azoulay Robin,
Olivier Paul,
Baud Olivier,
Verney Catherine,
Santus Robin,
Robert Philippe,
Gressens Pierre,
Sebag Guy
Publication year - 2008
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21510
Subject(s) - spleen , bone marrow , mononuclear phagocyte system , biodistribution , in vivo , chemistry , pathology , peritoneum , medicine , nuclear medicine , in vitro , biology , biochemistry , microbiology and biotechnology
Purpose To investigate whether USPIO‐enhanced magnetic resonance imaging (MRI) detected reticuloendothelial system (RES) cells in newborn normal rats. Materials and Methods Newborn normal rats were imaged in vivo on a 1.5 T MR system, 2–96 hours after intraperitoneal Ferumoxtran‐10 ( n = 38) or saline injection (control group, n = 5). Signals from liver, spleen, and vertebral bone marrow were measured (T2‐weighted Turbo Spin Echo) to describe the kinetics of enhancement. The pups were sacrificed and iron concentrations in plasma and peritoneal fluid were measured using atomic absorption spectrometry. Prussian blue‐labeled cells density in liver, spleen, and vertebral bone marrow was assessed. Results Significant ( P < 0.05) negative enhancement of the liver, spleen, and vertebral bone marrow was noted after Ferumoxtran‐10 injection (2–96 hours for liver and spleen, 4–96 hours for bone marrow). Ferumoxtran‐10 was absorbed from the peritoneum in the first 8 hours postinjection, entering the circulation with a plasma peak (8 hours); then Ferumoxtran‐10 returned over the baseline in plasma (96 hours). Important intracellular iron deposition in liver and spleen was measured postinjection (3–96 hours, P < 0.05). Limited but significant intracellular iron deposition was noted in vertebral bone marrow postinjection (96 hours, P < 0.05), suggesting that Ferumoxtran‐10 selectively labeled RES cells after 96 hours and produced nonspecific labeling at earlier timepoints. Conclusion Ferumoxtran‐10‐enhanced MRI visualizes RES cells in vivo in newborn rats. J. Magn. Reson. Imaging 2008;28:1046–1052. © 2008 Wiley‐Liss, Inc.