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Dynamic contrast‐enhanced (DCE) derived transfer coefficient (k trans ) is a surrogate marker of matrix metalloproteinase 9 (MMP‐9) expression in brain tuberculomas
Author(s) -
Haris Mohammad,
Husain Nuzhat,
Singh Anup,
Awasthi Rishi,
Singh Rathore Ram Kishore,
Husain Mazhar,
Gupta Rakesh K.
Publication year - 2008
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21491
Subject(s) - nuclear medicine , perfusion , medicine , microvessel , vascular endothelial growth factor , pathology , perfusion scanning , matrix metalloproteinase , lesion , white matter , blood volume , magnetic resonance imaging , chemistry , vegf receptors , immunohistochemistry , radiology
Purpose To correlate dynamic contrast‐enhanced (DCE) MRI derived perfusion indices with immunohistochemically obtained vascular endothelial growth factor (VEGF) and matrix metalloproteinase‐9 (MMP‐9) in a cellular fraction of brain tuberculomas (BT). Materials and Methods Thirteen BT patients underwent DCE MRI. Perfusion indices (cerebral blood volume [CBV], transfer coefficient [k trans ] and leakage [v e ]) maps were generated for the quantitative analysis. The CBV was corrected for the leaky blood–brain barrier (BBB). The relative CBV (rCBV), k trans , and v e were calculated by placing 10 regions of interest (ROIs) showing the highest values in the lesion. The percentage area of VEGF and percentage area of MMP‐9 and microvessel density (MVD) were quantified from 10 fields per lesion with maximal expression of the excised BT. Pearson correlation analysis between physiological indices and quantitative VEGF, MMP‐9, and MVD was performed for each ROI. Results The average value of rCBV, k trans , v e , VEGF, MMP‐9, and MVD were 3.53 ± 0.37, 2.04 ± 0.40 min −1 , 0.71 ± 0.09, 12.51 ± 2.56, 18.09 ± 2.06, 10.87 ± 1.99, respectively. The k trans ( r = 0.918, P < 0.001) and v e ( r = 0.899, P < 0.001) showed significant correlation with MMP‐9, while rCBV correlated significantly with MVD ( r = 0.962, P < 0.001) and VEGF ( r = 0.868, P < 0.001). Conclusion We conclude that the expression of MMP‐9, a marker of BBB disruption and disease activity in BT correlates with DCE‐derived k trans and thus has the potential to be used as its surrogate marker. J. Magn. Reson. Imaging 2008;28:588–597. © 2008 Wiley‐Liss, Inc.