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Metabolite signature of developmental foregut cyst on in vivo and in vitro 1 H MR spectroscopy
Author(s) -
Santhosh Kannath,
Thomas Bejoy,
Varma Luxmi,
Sandhyamany S.,
Kesavadas Chandrasekharan,
Appukuttan P.S.,
Srinivas G.,
Gupta Arun Kumar,
Kapilamoorthy T.R.,
Unnikrishnan M.
Publication year - 2008
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21428
Subject(s) - foregut , cyst , biology , in vivo , metabolite , respiratory epithelium , in vitro , pathology , chemistry , anatomy , nuclear magnetic resonance , biochemistry , respiratory system , medicine , physics , microbiology and biotechnology
Foregut duplication cysts are developmental anomalies of the bronchopulmonary foregut and are common cystic lesions of the mediastinum. We describe a case of mediastinal foregut duplication cyst with in vivo 1 H MR spectroscopy on a 1.5T magnet showing a large metabolite peak at 2.02 ppm, attributable to N‐acetylated compounds, in addition to a smaller peak at 1.33 ppm, considered to represent lipids. In vitro NMR spectroscopy (7.05T) of cyst fluid confirmed the presence of these peaks. In addition, a broad multiplet centered at 3.7 ppm, possibly from various protons of the hexose ring system, was also noted. Chemical analysis of the cyst fluid demonstrated the presence of N‐acetylhexosamines, proteins, and lipids. Again, in vitro spectra of pure samples of N‐acetylglucosamine and N‐acetylgalactosamine were obtained for comparison, which better resolved the N‐acetyl peak and the peaks at 3.7 ppm. The mucus secreted by respiratory epithelium and the mucous glands of the foregut cysts contains glycoproteins that have N‐acetylhexosamines as components and lipid breakdown products that are thought to contribute to the observed spectrum. This information might be useful in predicting the cyst content and, in turn, the lining of the epithelium and the glandular elements. J. Magn. Reson. Imaging 2008;28:493–496. © 2008 Wiley‐Liss, Inc.