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Detection of DNA double‐strand breaks using γh2AX after MRI exposure at 3 Tesla: An in vitro study
Author(s) -
Schwenzer Nina F.,
Bantleon Rüdiger,
Maurer Brigitte,
Kehlbach Rainer,
Schraml Christina,
Claussen Claus D.,
Rodegerdts Enno
Publication year - 2007
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21138
Subject(s) - flow cytometry , in vitro , dna , magnetic resonance imaging , microbiology and biotechnology , nuclear magnetic resonance , magnetostatics , cell culture , histone , double strand , magnetic field , chemistry , nuclear medicine , biology , dna damage , medicine , physics , biochemistry , genetics , quantum mechanics , radiology
Purpose To evaluate the effects of the static magnetic field and typical imaging sequences of a high‐field magnetic resonance scanner (3 Tesla) on the induction of double‐strand breaks (DSBs) in two different human cell lines. Materials and Methods Human promyelocytic leukemia cells (HL‐60) and human acute myeloid leukemia cells (KG‐1a) were exposed to the static magnetic field alone and to turbo spin‐echo (TSE) and gradient‐echo (GE) sequences. Flow cytometry was used to quantify γH2AX (serine 139 phosphorylated form of histone H2AX) expression of antibody‐stained cells as a marker for deoxyribonucleic acid (DNA) DSBs one hour and 24 hours after magnetic field exposure. X‐ray–treated cells were used as positive control. Results Neither exposure to the static magnetic field alone nor to the applied imaging sequences showed significant differences in γH2AX expression between exposed and sham‐exposed cells. X‐ray–treated cells as positive control showed a significant increase in γH2AX expression. Conclusion The static magnetic field alone and MRI sequences at 3 Tesla have no effect on the induction of DSBs in HL‐60 and KG‐1a cells. J. Magn. Reson. Imaging 2007;26:1308–1314. © 2007 Wiley‐Liss, Inc.