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Iron‐oxide labeling of hematogenous macrophages in a model of experimental autoimmune encephalomyelitis and the contribution to signal loss in fast imaging employing steady state acquisition (FIESTA) images
Author(s) -
Oweida Ayman J.,
Dunn Elizabeth A.,
Karlik Stephen J.,
Dekaban Gregory A.,
Foster Paula J.
Publication year - 2007
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.21005
Subject(s) - experimental autoimmune encephalomyelitis , magnetic resonance imaging , pathology , medicine , encephalomyelitis , in vivo , multiple sclerosis , radiology , biology , immunology , disease , microbiology and biotechnology
Purpose To determine the contribution of blood‐derived macrophages to the signal loss observed in MR images of inflammatory lesions in experimental autoimmune encephalomyelitis (EAE). Materials and Methods A relapsing‐remitting form of EAE was induced in transgenic mice that express enhanced green fluorescent protein (EGFP) specifically in hematopoietic cells of the myelomonocytic lineage. Animals were injected with Feridex, a superparamagnetic iron oxide (SPIO) nanoparticle, 24 hours prior to in vivo MRI. MRI was performed using a 1.5T whole‐body scanner; a high‐performance, custom‐built gradient coil insert; and a 3D steady‐state free precession (SSFP) imaging pulse sequence. Comparisons were made between MR images and corresponding anti‐GFP and Perl's Prussian blue (PPB)‐stained brain sections. Results MR images revealed the presence of discrete regions of signal loss throughout the brains of EAE animals that were administered Feridex. Histological staining showed that regions of signal loss on MR images corresponded anatomically with regions of PPB‐ and GFP‐positive cells. Conclusion This experiment provides the first direct evidence that macrophages of hematogenous origin are labeled with SPIO after intravenous administration of Feridex, and contribute to the regions of signal loss detected in MR images of EAE brain. J. Magn. Reson. Imaging 2007;26:144–151. © 2007 Wiley‐Liss, Inc.