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Intramyocellular lipid quantification from 1 H long echo time spectra at 1.5 and 3 T by means of the LCModel technique
Author(s) -
Škoch Antonín,
Jírů Filip,
Dezortová Monika,
Krušinová Eva,
Kratochvílová Simona,
Pelikánová Terezie,
Grodd Wolfgang,
Hájek Milan
Publication year - 2006
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.20574
Subject(s) - reproducibility , insulin resistance , chemistry , diabetes mellitus , medicine , endocrinology , chromatography
Purpose To introduce a method of independent determination of CH 2 and CH 3 components of intramyocellular lipids (IMCLs) by using long TE for spectra measurement and LCModel for spectra evaluation, to test this technique in controls and insulin‐resistant subjects, and to compare results at 1.5 and 3 T. Materials and Methods Eight healthy volunteers and 11 patients with type 2 diabetes mellitus underwent measurement using a 1.5‐T MR scanner; six healthy volunteers were measured using a 3‐T MR scanner. Spectra from the tibialis anterior muscle were acquired by using a point resolved spectroscopy (PRESS) sequence with the following parameters: TR/TE/ACQ = 2000 msec/270 msec/256. Spectra were processed by LCModel 6.1 software with two types of adopted basis‐set. Results Spectra with good separation of both CH 2 and CH 3 components of IMCL and extramyocellular lipids (EMCLs) were obtained and the LCModel routine was successfully applied. The reproducibility comparison ( N = 7 at 1.5 T vs. N = 5 at 3 T) showed that better results can be obtained at higher B 0 values. The comparison of the healthy and insulin‐resistant subjects proved that both IMCL_CH 2 /Cr and IMCL_CH 3 /Cr ratios significantly differ. Conclusion Long TE spectroscopy of the human muscle with IMCL quantification using the LCModel technique can detect changes in IMCL levels as well as help in the study of fatty acyl chain composition. Using a higher field strength increased the intra‐individual reproducibility by approximately 150% J. Magn. Reson. Imaging 2006. © 2006 Wiley‐Liss, Inc.