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Variations due to analysis technique in intracellular pH measurements in simulated and in vivo 31 P MR spectra of the human brain
Author(s) -
Hamilton Gavin,
Allsop Joanna M.,
Patel Nayna,
Forton Daniel M.,
Thomas Howard C.,
O'Sullivan Catherine P.A.,
Hajnal Joseph V.,
TaylorRobinson Simon D.
Publication year - 2006
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.20524
Subject(s) - in vivo , phosphocreatine , spectral line , intracellular ph , nuclear magnetic resonance , chemistry , analytical chemistry (journal) , accuracy and precision , biomedical engineering , materials science , mathematics , physics , chromatography , intracellular , medicine , statistics , biology , biochemistry , microbiology and biotechnology , astronomy , energy metabolism
Purpose To investigate variation in pH generated by different analysis techniques and to find the most robust method, 31 P MR brain spectra were acquired in vivo. Three different methods were used to measure the chemical shift of inorganic phosphate (Pi) relative to phosphocreatine (PCr). Materials and Methods Eight healthy volunteers were scanned four times, and manual measurement of the chemical shift in a frequency domain spectrum using the manufacturer's software was compared with values produced by a frequency‐domain analysis method (NMR1) and a prior‐knowledge‐based time‐domain technique (MRUI). To explain the in vivo data, simulations of brain spectra, modified in ways typical of real variations in vivo, were produced and the pH was measured using manual measurement and MRUI. Results Different measurement techniques produced systematically different pH values, with manual measurement producing the lowest variability (manual measurement: pH = 6.999, CoV = 0.297; NMR1: pH = 7.042, CoV = 0.501; MRUI: pH = 7.036, CoV = 0.606). While MRUI more accurately measured the pH of unaltered simulations, it was systematically affected by altering the simulated spectra. Manual measurement was unaffected. Conclusion Manual measurement produces the most consistent pH value, and there is no benefit in using more complex automated spectral fitting methods to measure the pH. J. Magn. Reson. Imaging 2006. © 2006 Wiley‐Liss, Inc.