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Cellular disorders induced by high magnetic fields
Author(s) -
Valiron Odile,
Peris Leticia,
Rikken Geert,
Schweitzer Annie,
Saoudi Yasmina,
Remy Chantal,
Job Didier
Publication year - 2005
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.20398
Subject(s) - cytoskeleton , viability assay , microbiology and biotechnology , cell , actin cytoskeleton , cell culture , cell adhesion , actin , biology , focal adhesion , cell growth , cellular differentiation , signal transduction , biochemistry , genetics , gene
Abstract Purpose To evaluate whether static high magnetic fields (HMFs), in the range of 10–17 T, affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons. Materials and Methods Cells were exposed to HMF for 30 or 60 minutes and subsequently assessed for viability. Cytoskeleton arrays and focal adhesions were visualized using immunofluorescence microscopy. Results Cell exposure to HMF over 10 T in the case of cycling cells, and over 15 T in the case of neurons, affected cell viability, apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, exposure to HMF induced growth cone retraction and delayed cell differentiation. Conclusion Cell exposure to HMF (over 10T and 15 T in the case of cycling cells and neurons, respectively) affects the cell cytoskeleton, with deleterious effects on cell viability, organization, and differentiation. Further studies are needed to determine whether such perturbations, as observed here in cultured cells, have consequences in whole animals. J. Magn. Reson. Imaging 2005. © 2005 Wiley‐Liss, Inc.