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Intratumor heterogeneity in blood perfusion in orthotopic human melanoma xenografts assessed by dynamic contrast‐enhanced magnetic resonance imaging
Author(s) -
Gaustad JonVidar,
Benjaminsen Ilana C.,
Graff Bjørn A.,
Brurberg Kjetil G.,
Ruud ElseBeate M.,
Rofstad Einar K.
Publication year - 2005
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.20321
Subject(s) - dynamic contrast , magnetic resonance imaging , perfusion , medicine , contrast (vision) , melanoma , dynamic contrast enhanced mri , perfusion scanning , nuclear magnetic resonance , radiology , pathology , magnetic resonance angiography , nuclear medicine , cancer research , computer science , physics , artificial intelligence
Purpose To determine the intratumor heterogeneity in blood perfusion of orthotopic human melanoma xenografts by use of gadopentetate dimeglumine (Gd‐DTPA)‐based dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI). Materials and Methods Orthotopic xenografts of an amelanotic human melanoma cell line (A‐07) were scanned sagittally, coronally, and axially in three subsequent DCE‐MRI sessions, using spoiled gradient recalled sequences, a voxel size of 0.31 × 0.62 × 2.0 mm 3 , and an interleaving acquisition method to avoid slice gaps. Tumor images of E · F ( E is initial extraction fraction and F is perfusion) were produced by subjecting the DCE‐MRI data to Kety analysis. E · F was used as a parameter for tumor blood perfusion, since E for Gd‐DTPA is close to unity in A‐07 tumors. Results All A‐07 tumors subjected to investigation showed anisotropic radial heterogeneity in blood perfusion. The blood perfusion was low in the center of the tumors and increased toward the tumor periphery in the cranial, dorsal, caudal, and ventral directions, but not in the lateral and medial directions. In addition, 9 of 10 tumors showed blood perfusion hot spots in central or nonperipheral regions. The hot spots differed significantly between tumors in size, shape, location, and intensity, and appeared to be governed by stochastic processes. This heterogeneity superimposed the radial heterogeneity, but did not overshadow it in any tumor. Conclusion Orthotopic human melanoma xenografts show significant intratumor heterogeneity in blood perfusion. This heterogeneity is made up of two distinctly different components, one stochastic and one nonstochastic radial component. The radial component is anisotropic and dominant and is superimposed by the stochastic component. J. Magn. Reson. Imaging 2005;21:792–800. © 2005 Wiley‐Liss, Inc.