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Long‐term imaging effects in rat liver after a single injection of an iron oxide nanoparticle based MR contrast agent
Author(s) -
BrileySæbø Karen,
Hustvedt SveinOlaf,
Haldorsen Anita,
Bjørnerud Atle
Publication year - 2004
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.20175
Subject(s) - in vivo , chemistry , pharmacokinetics , iron oxide , ex vivo , half life , nuclear medicine , medicine , pharmacology , in vitro , biochemistry , biology , microbiology and biotechnology , organic chemistry
Purpose To investigate the duration of liver R2* enhancement and pharmacokinetics following administration of an iron oxide nanoparticle in a rat model. Materials and Methods Rats were injected with 0, 1, 2, or 5 mg Fe/kg of NC100150 Injection, and quantitative in vivo 1/T2* liver measurements were obtained between 1 and 133 days after injection. The concentration of NC100150 Injection was determined by relaxometry methods in ex vivo rat liver homogenate. Results At all dose levels, 1/T2* remained greater than control values up to 63 days after injection. In the highest dose group, 1/T2* was above control levels during the entire 133 day time‐course investigated. There were no quantifiable amounts of NC100150 Injection present 63 days after injection in any of the dose groups. The half‐life of NC100150 Injection in rat liver was dose dependent. For the lowest dose group, the degradation of the particles could be defined by a mono‐exponential function with a half‐life of eight days. For the 2 and 5 mg Fe/kg dose groups, the degradation was bi‐exponential with a fast initial decay of seven to eight days followed by a slow terminal decay of 43–46 days. Conclusion NC100150 Injection exhibits prolonged 1/T2* enhancement in rat liver. The liver enhancement persisted at time points when the concentration of iron oxide particles present in the liver was below method detection limits. The prolonged 1/T2* enhancement is likely a result of the particle breakdown products and the induction of ferritin and hemosiderin with increasing iron cores/loading factors. J. Magn. Reson. Imaging 2004;20:622–631. © 2004 Wiley‐Liss, Inc.