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Interaction between paramagnetic metal complexes and intracellular water of single cells
Author(s) -
Päuser Sabine,
Zschunke Adolf,
Keller Konrad
Publication year - 1996
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.1880060144
Subject(s) - xenopus , intracellular , paramagnetism , cytoplasm , relaxation (psychology) , chemistry , membrane , water transport , biophysics , metal , chelation , nuclear magnetic resonance , metal ions in aqueous solution , ion , inorganic chemistry , biochemistry , biology , physics , organic chemistry , quantum mechanics , neuroscience , water flow , environmental engineering , engineering , gene
Oocytes from Xenopus laevis were used as a well‐established model to investigate spatially resolved changes in relaxation time (T1) within a cell during exposure to copper complexes by means of 'H‐NMR‐microscopy. T1 relaxation of intracellular water was shortened in dependence on the complex concentration, the water content, and the water mobility in various cell compartments. A relatively constant T1 decrease was observed in the cytoplasm of the vegetal pole, irrespective of the type and concentration of the permeable complexes used. Since the lowest content of free mobile water molecules was detected at the vegetal pole, we concluded that this quantity was as important for the relaxation time decrease as that of the chelated paramagnetic ions. Experiments using 14 C‐labeled inulin demonstrated that the paramagnetic metal complexes entered the oocytes without gross injury to their plasma membranes.