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Correlation of MR imaging and histologic findings in mouse melanoma
Author(s) -
DeJordy John O.,
Bendel Peter,
Salomon Yoram,
Degani Hadassa,
Horowitz Ada
Publication year - 1992
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.1880020614
Subject(s) - melanin , melanoma , magnetic resonance imaging , correlation , nuclear magnetic resonance , intensity (physics) , pathology , nuclear medicine , medicine , chemistry , radiology , physics , optics , mathematics , biochemistry , geometry , cancer research
M2R melanoma tumors in male C57 black mice were used to correlate magnetic resonance (MR) images with the corresponding histologic slices and to determine if analysis of the achievable correlation can provide a basis for predicting gross histologic features with MR imaging alone. The MR imaging sections obtained at 4.7 T were each 680 μm thick, with an in‐plane resolution of 195 μm. The distribution of melanin within the histologic slices correlated well with the high‐signal‐intensity regions on the T1‐weighted images (T1W1s), while these regions had low signal intensity on the T2‐weighted images (T2WIs), providing evidence that melanin or melanin‐associated paramagnetic species are responsible for the observed proton relaxation rate enhancement. Viable melanoma cells typically showed intermediate signal intensity on T2WIs, T1WIs, and proton‐density images. Necrosis typically had high signal intensity on T2WIs, T1WIs, and proton‐density images. Quantitation of the MR imaging results, followed by statistical analysis, demonstrated statistically significant differences between melanin‐rich, viable‐melanoma, and necrotic regions on MR images.