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MR microscopy of chick embryo vasculature
Author(s) -
Smith Bradley R.,
Effmann Eric L.,
Allan Johnson G.
Publication year - 1992
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.1880020220
Subject(s) - embryo , microscopy , anatomy , biomedical engineering , biology , pathology , medicine , microbiology and biotechnology
Six‐day‐old chick embryos were examined with magnetic resonance microscopy after vascular perfusion fixation and perfusion with gadolinium‐doped gelatin to highlight the developing vascular anatomy. Gadolinium gelatin, with its short T1, provided a source of signal contrast within the vessels. The entire embryo was embedded in gelatin to minimize susceptibility artifacts that are prevalent at the high field strength (7.0 T) used. A series of single‐section spin‐echo images were acquired with various TRs to determine the optimal imaging sequence for a three‐dimensional (3D) acquisition. The combination of gadolinium gelatin in the vascular spaces, gelatin embedding of the specimen, and optimal acquisition parameters yielded a 3D stack of high‐resolution images that was readily reconstructed and rendered to effectively demonstrate the developing thoracic vessels in the embryo.