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Radio‐frequency‐induced thermal lesions: Subacute magnetic resonance appearance and histological correlation
Author(s) -
Lazebnik Roee S.,
Breen Michael S.,
Fitzmaurice Maryann,
Nour Sherif G.,
Lewin Jonathan S.,
Wilson David L.
Publication year - 2003
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.10382
Subject(s) - magnetic resonance imaging , h&e stain , anatomy , histology , pathology , masson's trichrome stain , nuclear magnetic resonance , medicine , materials science , nuclear medicine , immunohistochemistry , physics , radiology
Purpose To investigate the relationship between subacute magnetic resonance (MR) images of radio‐frequency (RF) ablation lesions and tissue viability as determined from histological tissue samples. Materials and Methods We generated lesions ( N = 5) in a rabbit thigh model. Four days later, we obtained in vivo T 2 ‐ and contrast‐enhanced (CE) T 1 ‐weighted images and ex vivo histological samples approximately perpendicular to the electrode path. Using three‐dimensional registration and warping, we spatially compared manually segmented boundaries apparent on MR images to boundaries separating distinct histological zones determined from hematoxylin and eosin (H&E) and Masson trichrome (MT) stains, as well as birefringence studies. Results Lesions have a characteristic MR appearance: an outer hyperintense margin (M2) separating background tissue (M3) from an inner core (M1), in both T 2 and CE T 1 images. Histologically, there are two zones of damage: an outer zone of likely nonviable cells (H2) separating background tissue (H3) from an inner core of coagulated nonviable cells (H1). We measured distances between automatically computed correspondence points along histological and MR boundaries. For T 2 and CE T 1 images, respectively, M1 vs. H1 distances were 0.72 ± 0.99 mm (mean ± SD) and 0.10 ± 0.95 mm, while outer M2 vs. H2 boundary distances were 0.26 ± 1.16 mm and 0.05 ± 1.08 mm. The discrepancy between histological and MR boundaries was larger than the variability in segmenting MR images, but probably within registration error. There were no significant differences between T 2 and CE T 1 boundaries. Conclusion Lesion boundaries apparent in both T 2 ‐ and CE T 1 ‐weighted MR scans, performed several days postablation, similarly predict the histological response. That is, the lesion core (M1) corresponds to nonviable coagulated cells (H1), while the hyperintense margin (M2) corresponds to likely nonviable cells undergoing necrotic changes (H2). J. Magn. Reson. Imaging 2003;18:487–495. © 2003 Wiley‐Liss, Inc.