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Functional imaging of the rat cervical spinal cord
Author(s) -
Malisza Krisztina L.,
Stroman Patrick W.
Publication year - 2002
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.10185
Subject(s) - spinal cord , medicine , magnetic resonance imaging , radiology , anatomy , psychiatry
Purpose: To examine functional magnetic resonance imaging (fMRI) of the rat cervical spinal cord using painful stimulation. Materials and Methods: fMRI of the rat cervical spinal cord was performed at 9.4 T. Stimuli included injection of 25μL of capsaicin (128 μg/mL in 7.5% dimethylsulfoxide (DMSO)) into the right dorsal forepaw and electrical stimulation (15 V, 0.3 msec, 3 Hz) of the left dorsal forepaw. Results: Activation in the dorsal horn of the spinal cord, which is known to be associated with the transmission of pain, was found in all rats ( N = 4) following injection of capsaicin into the dorsal forepaw. It was possible to reproduce the pain response in a given animal several times throughout the course of an experiment, provided that sufficient time was allowed between capsaicin injections. Regions of the spinal cord associated with motor and pain response were observed in functional imaging experiments involving subcutaneous electrical stimulation of the dorsal forepaw. Conclusion: Spinal fMRI using electrical stimulation and capsaicin‐induced painful stimulation can be a useful tool in an animal model of pain and injury. J. Magn. Reson. Imaging 2002;16:553–558. © 2002 Wiley‐Liss, Inc.