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Atomic force microscopy for cellular level manipulation: imaging intracellular structures and DNA delivery through a membrane hole
Author(s) -
Afrin Rehana,
Zohora Umme Salma,
Uehara Hironori,
WatanabeNakayama Takahiro,
Ikai Atsushi
Publication year - 2009
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.971
Subject(s) - transfection , fluorescence microscope , biophysics , green fluorescent protein , intracellular , gene delivery , nanotechnology , atomic force microscopy , fluorescence lifetime imaging microscopy , cell membrane , fluorescence , sonoporation , microscopy , cell , chemistry , microbiology and biotechnology , materials science , biology , gene , biochemistry , optics , physics , microbubbles , acoustics , ultrasound
The atomic force microscope (AFM) is a versatile tool for imaging, force measurement and manipulation of proteins, DNA, and living cells basically at the single molecular level. In the cellular level manipulation, extraction, and identification of mRNA's from defined loci of a cell, insertion of plasmid DNA and pulling of membrane proteins, for example, have been reported. In this study, AFM was used to create holes at defined loci on the cell membrane for the investigation of viability of the cells after hole creation, visualization of intracellular structure through the hole and for targeted gene delivery into living cells. To create large holes with an approximate diameter of 5–10 µm, a phospholipase A 2 coated bead was added to the AFM cantilever and the bead was allowed to touch the cell surface for approximately 5–10 min. The evidence of hole creation was obtained mainly from fluorescent image of Vybrant DiO labeled cell before and after the contact with the bead and the AFM imaging of the contact area. In parallel, cells with a hole were imaged by AFM to reveal intracellular structures such as filamentous structures presumably actin fibers and mitochondria which were identified with fluorescent labeling with rhodamine 123. Targeted gene delivery was also attempted by inserting an AFM probe that was coated with the Monster Green Fluorescent Protein phMGFP Vector for transfection of the cell. Following targeted transfection, the gene expression of green fluorescent protein (GFP) was observed and confirmed by the fluorescence microscope. Copyright © 2009 John Wiley & Sons, Ltd.