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Immunization of rabbits with DNase I produces polyclonal antibodies with DNase and RNase activities
Author(s) -
Krasnorutskii Michael A.,
Buneva Valenti.,
Nevinsky Georgy A.
Publication year - 2008
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.890
Subject(s) - polyclonal antibodies , rnase p , dna , affinity chromatography , microbiology and biotechnology , rna , antibody , chemistry , deoxyribonuclease i , biochemistry , sepharose , enzyme , deoxyribonuclease , biology , immunology , gene , base sequence
Immunization of rabbits with DNase I leads to the production of antiidiotypic Abs with DNase activity. It is not known at present whether antiidiotypic Abs against DNA‐hydrolyzing enzymes can possess RNase activity. Here we show that immunization of healthy rabbits with bovine DNase I produces IgGs with intrinsic DNase and RNase activities. Electrophoretically and immunologically homogeneous polyclonal IgGs were obtained by sequential chromato‐graphy of the immune sera on Protein A‐Sepharose and gel filtration. Affinity chromatography on DNA cellulose using elution of Abs with different concentrations of NaCl and an acidic buffer separated catalytic IgGs into four Ab subfractions, three of which demonstrated only DNase activity while one subfraction hydrolyzed RNA faster than DNA. The serum of patients with many different autoimmune (AI) diseases contains small fractions of antibodies (Abs) interacting with immobilized DNA, which possess both DNase and RNase activities. Our data suggest that a fraction of abzymes from AI patients hydrolyzing both DNA and RNA can contain a subfraction of Abs against DNase I. Copyright © 2008 John Wiley & Sons, Ltd.