Nucleic acid sensor for insecticide detection | Zendy

Solanki Pratima R. | Zendy; Prabhakar Nirmal | Zendy; Pandey M. K. | Zendy; Malhotra B. D. | Zendy

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Nucleic acid sensor for insecticide detection

Author(s) -

Solanki Pratima R.,

Prabhakar Nirmal,

Pandey M. K.,

Malhotra B. D.

Publication year - 2008

Publication title -

journal of molecular recognition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.401

H-Index - 79

eISSN - 1099-1352

pISSN - 0952-3499

DOI - 10.1002/jmr.888

Subject(s) - polyaniline , fourier transform infrared spectroscopy , cyclic voltammetry , nuclear chemistry , nucleic acid , chemistry , hydrochloride , covalent bond , scanning electron microscope , indium tin oxide , biosensor , materials science , electrochemistry , electrode , organic chemistry , chemical engineering , polymer , biochemistry , composite material , engineering , polymerization

Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO − 4 )‐doped PANI film deposited onto indium‐tin‐oxide (ITO) glass plate using 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride (EDC)/ N ‐hydroxysuccinimide (NHS) chemistry. These dsCT‐DNA‐PANI‐ClO 4 /ITO and PANI‐ClO 4 /ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier‐transform‐infrared (FTIR) measurements. This disposable dsCT‐DNA‐PANI‐ClO 4 /ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005 ppm) and trichlorfon (0.01 ppm) in 30 and 60 s, respectively. Copyright © 2008 John Wiley & Sons, Ltd.

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