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Recombinant shark natural antibodies to thyroglobulin
Author(s) -
Schluter Samuel F.,
Jensen Ingvill,
Ramsland Paul A.,
Marchalonis John J.
Publication year - 2005
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.742
Subject(s) - thyroglobulin , recombinant dna , antibody , natural (archaeology) , chemistry , biochemistry , biology , immunology , gene , paleontology
As cartilaginous fish are the vertebrates most distal from man to produce antibodies, fundamental information regarding conservation and variation of the antigen binding site should be gained by comparing the properties of antibodies directed against the same antigen from the two species. Since monoclonal cell lines cannot be generated using shark B cells, we isolated antigen binding recombinant single chain Fv antibodies (scFv) comprising of the complete variable regions from shark light and heavy chains. Thyroglobulin was used as the selecting antigen as both sharks and humans express natural antibodies to mammalian thyroglobulin in the absence of purposeful immunization. We report that recombinant sandbar shark ( Carcharhinus plumbeus ) scFvs that bind bovine thyroglobulin consist of heavy chain variable regions (VH) homologous to those of the human VHIII subset and light chain variable regions (VL) homologous to those of the human Vλ6 subgroup. The homology within the frameworks is sufficient to enable the building of three‐dimensional models of the shark VH/VL structure using established human structures as templates. In natural antibodies of both species, the major variability lies in the third complementarity determining region (CDR3) of both VH and VL. Copyright © 2005 John Wiley & Sons, Ltd.