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Non‐ideal tracer sedimentation equilibrium: a powerful tool for the characterization of macromolecular interactions in crowded solutions
Author(s) -
Rivas Germán,
Minton Allen P.
Publication year - 2004
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.708
Subject(s) - macromolecular crowding , macromolecule , tracer , ideal (ethics) , sedimentation , a priori and a posteriori , sedimentation equilibrium , ideal solution , experimental data , characterization (materials science) , statistical physics , chemistry , biochemical engineering , biological system , mathematics , materials science , nanotechnology , chromatography , statistics , physics , geology , biology , epistemology , philosophy , engineering , ultracentrifuge , paleontology , biochemistry , nuclear physics , sediment
Non‐ideal tracer sedimentation equilibrium is a technique devised to quantify the effect of high concentrations of unrelated macromolecules on the self‐ or hetero‐associations of dilute macromolecules. Principles and experimental techniques are reviewed, and previous experimental work summarized. A new analysis of experimental data is presented that requires no a priori assumptions regarding the nature of weak repulsive interactions between solute species and the concentrated (crowding) species. Published in 2004 by John Wiley & Sons, Ltd.