Premium
Advances in membrane receptor screening and analysis
Author(s) -
Cooper Matthew A.
Publication year - 2004
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.675
Subject(s) - chemistry , receptor , computational biology , biochemistry , biology
During the last decade there has been significant progress in the development of analytical techniques for the screening of ligand binding to membranes and membrane receptors. This review focuses on developments using label‐free assays that facilitate ligand–membrane–receptor screening without the need for chemical‐, biological‐ or radiological‐labelled reagents. These assays include acoustic, optical surface plasmon resonance biosensing, sedimentation (analytical ultracentrifugation), chromatographic assays, isothermal titration calorimetry and differential scanning calorimetry. The merits and applications of cell‐based screening systems and of different model membrane systems, including planar supported lipid layers, bead‐supported membranes and lipid micro‐arrays, are discussed. Recent advances involving more established techniques including intrinsic fluorescence, FRET spectroscopy, scintillation proximity assays and automated patch clamping are presented along with applications to peripheral membrane proteins, ion channels and G protein‐coupled receptors. Novel high‐throughput assays for determination of drug‐ and protein‐partitioning in membranes are also highlighted. To aid the experimenter, a brief synopsis of the techniques commonly employed to purify and reconstitute membranes and membrane receptors is included. Copyright © 2004 John Wiley & Sons, Ltd