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Molecular tools for a molecular medicine: analyzing genes, transcripts and proteins using padlock and proximity probes
Author(s) -
Landegren Ulf,
Schallmeiner Edith,
Nilsson Mats,
Fredriksson Simon,
Banér Johan,
Gullberg Mats,
Jarvius Jonas,
Gustafsdottir Sigrun,
Dahl Fredrik,
Söderberg Ola,
Ericsson Olle,
Stenberg Johan
Publication year - 2004
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.664
Subject(s) - computational biology , encode , rolling circle replication , nucleic acid , dna , gene , genome , ligation , chemistry , biology , biochemistry , microbiology and biotechnology , polymerase
Procedures and reagents are needed to specifically detect all the macromolecules that are being identified in the course of genome projects. We discuss how this challenge may be met using a set of ligation‐based reagents termed padlock probes and proximity ligation probes. These probes include elements with affinity for specific nucleic acid and protein molecules, respectively, along with unique identifier DNA sequence elements that encode the identity of the recognized target molecules. The information content of DNA strands that form in the detection reactions are recorded after amplification, allowing the recognized target molecules to be identified. The procedures permit highly specific solution‐phase or localized analyses of large sets of target molecules as required in future molecular analyses. Copyright © 2004 John Wiley & Sons, Ltd.