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Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants
Author(s) -
SánchezSancho Francisco,
PerezInestrosa Ezequiel,
Suau Rafael,
Montañez María I.,
Mayorga Cristobalina,
Torres Maria J.,
Romano Antonino,
Blanca Miguel
Publication year - 2003
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.621
Subject(s) - cephalosporin , antigen , cephalosporin antibiotic , chemistry , antibiotics , biochemistry , antibody , combinatorial chemistry , epitope , molecule , computational biology , biology , immunology , organic chemistry
Lack of knowledge of the exact chemical structure of cephalosporin antigenic determinants has hindered clinical interpretation of adverse reactions to these drugs and delayed understanding of the mechanisms involved in the specific recognition and binding of IgE molecules to these antigenic determinants. We further resolve the relationship between structure and activity of proposed antigenic chemicals, including the rational design and synthesis of these haptenic structures. Comparative RAST inhibition studies of the synthesized molecules revealed that they were recognized by IgE antibodies induced by cephalosporin antibiotics. Thus, these data indicate that recognition is mainly directed to the acyl side chain and to the β‐lactam fragment that remains linked to the carrier protein in the cephalosporin conjugation course. Copyright © 2003 John Wiley & Sons, Ltd.