Premium
Anti‐β 2 ‐glycoprotein I antibodies recognizing platelet factor 4–heparin complex in antiphospholipid syndrome in patient substantiated with mouse Model
Author(s) -
Bourhim Mustapha,
Darnige Luc,
Legallais Cécile,
Arvieux Josiane,
Cevallos Ramiro,
Pouplard Claire,
Vijayalakshmi Mookambeswaran A.
Publication year - 2003
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.619
Subject(s) - antiphospholipid syndrome , platelet factor 4 , antibody , platelet , glycoprotein , immunology , heparin induced thrombocytopenia , heparin , affinity chromatography , platelet activation , medicine , chemistry , endocrinology , biochemistry , enzyme
The antiphospholipid syndrome is defined by the presence of antiphospholipid antibodies associated with arterial and/or venous thrombosis, and recurrent abortion accompanied often by thrombocytopenia. These antibodies are heterogeneous and react against phospholipid‐binding proteins such as β 2 ‐glycoprotein I (β 2 GPI) and prothrombin. The recognition of anti‐β 2 ‐glycoprotein I (anti‐β 2 GPI) by platelet factor 4–heparin complex (PF4–Hc) has been previously evoked and partially confirmed by the present inhibition studies. Further, the anti‐β 2 ‐glycoprotein I antibodies were purified from a patient with primary antiphospholipid syndrome using Affi‐gel®‐10‐β 2 GPI immunoaffinity chromatography. The purified anti‐β 2 GPI IgM as well as patient serum equally recognized PF4–Hc in ELISA mode. In order to substantiate this data and to better understand we studied an animal model using mouse active immunization with the purified human anti‐β 2 GPI. The mice showed a significant decrease in their platelet count. In addition the ELISA responses of the immunized mice sera were positive against both β 2 GPI and PF4–Hc, substantiating the double recognition. Despite many previous reported animal model studies, this is the first time we have shown the specific recognition of anti‐β 2 GPI antibodies by PF4–Hc, the results in the induced mice correlating the data observed with some patients. Copyright © 2003 John Wiley & Sons, Ltd.