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The use of the resonant mirror biosensor to detect point mutations, as demonstrated with synthetic oligonucleotides
Author(s) -
Momynaliev K. T.,
Govorun V. M.,
Gnedenko O.,
Ivanov Yu. D.,
Archakov A. I.
Publication year - 2003
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.580
Subject(s) - point mutation , biosensor , oligonucleotide , mutant , 23s ribosomal rna , nucleotide , genetics , chemistry , gene , biophysics , biology , microbiology and biotechnology , materials science , nanotechnology , rna , ribosome
The possibility of using the resonant mirror biosensor to detect point substitutions in oligonucleotides was demonstrated with a fragment of the Helicobacter pylori 23S rRNA gene, point mutations in which are responsible for clarythromycin resistance. Conditions were optimized for the interaction of a probe immobilized on the sensing surface with targets containing various nucleotide substitutions. A probe allowing reliable discrimination of mutant targets was selected. The mismatch position in the probe was shown to affect the kinetic parameters (response) of hybridization with mutant targets, reporting not only the position, but also the character (G or C) of a substitution. Copyright © 2003 John Wiley & Sons, Ltd.