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BIACORE J: a new platform for routine biomolecular interaction analysis †
Author(s) -
Rich Rebecca L.,
Myszka David G.
Publication year - 2001
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.535
Subject(s) - binding affinities , chemistry , biosensor , computational biology , nanotechnology , receptor , biochemistry , biology , materials science
Abstract SPR biosensor technology continues to evolve. The recently released platform from Biacore AB (Uppsala, Sweden), BIACORE J, is designed for the routine analysis of biomolecular interactions. Using an antibody–protein A and a ligand–receptor system, we demonstrate the utility of BIACORE J in determining active concentration and binding affinities. The results from these studies illustrate the high sensitivity of the instrument and its ability to generate reproducible binding responses. The BIACORE J is easy to operate and useful in diverse applications, making SPR technology widely accessible as a research tool. Copyright © 2001 John Wiley & Sons, Ltd.