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Importance of the matrix and its chemical activation on the stability of immuno‐adsorbents
Author(s) -
Ubrich N.,
Hubert P.,
Dellacherie E.,
Rivat C.
Publication year - 1995
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.300080120
Subject(s) - adsorption , matrix (chemical analysis) , chemistry , chemical stability , chemical engineering , biochemical engineering , pulp and paper industry , environmental chemistry , chromatography , organic chemistry , engineering
Various matrices were reacted with four different activation reagents, in order to prepare immuno‐adsorbents for the selective removal of low‐density lipoproteins from blood plasma of patients affected by hypercholesterolaemia. The resulting immuno‐adsorbents were compared to that obtained earlier with cyanogen bromide activation, in terms of coupling yield, adsorption capacity and moreover stability towards the various chemical and biochemical conditions to which they are submitted during their handling. Tresyl chloride‐activated Sepharose 6 Fast Flow turns out to afford optimal stability in the whole pH range tested.