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Strong immunogenicity of a multicomponent peptide vaccine developed with the branched lysine oligopeptide method for human immunodeficiency virus infection
Author(s) -
Okuda Kenji,
Kaneko Tamiko,
Yamakawa Tadashi,
Tanaka Shunichi,
Shigematsu Takashi,
Yamamoto Akihiro,
Hamajima Kenji,
Nakajima Kiichiro,
Kawamoto Susumu,
Phanuphak Praphan
Publication year - 1993
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.300060302
Subject(s) - immunogenicity , peptide , oligopeptide , adjuvant , antiserum , peptide vaccine , epitope , virology , microbiology and biotechnology , immunization , chemistry , antigen , biology , biochemistry , immunology
We synthesized one V3 peptide each from HTLV‐III B , Thai A and Thai B, conjugating then to the T cell epitope of the env region, and we also synthesized a p17 protein peptide of the gag region (HGP‐30). These peptide were then coupled to 8‐lysine copolymers using N ‐succinimidyl maleimido carboxylate ( M r = ca 60 000). We designated this the branched lysine oligopeptide method. The large peptide complexes constructed from these four macromolecular peptide were used with aluminium hydroxide or complete Freund's adjuvant to immunize mice and rabbits four times. ELISA assay with aluminium hydroxide or complete Freund's adjuvant to immunize mice and rabbits four times. ELISA assay showed high titres of anti‐peptide antibodies to each V3loop peptide and the HGP‐30 peptide. Strong inhibition of CD4 + dependent cell fusion was obtained with these antisera when III b , Thai A and Thai B strains of human immunodeficiency virus (HIV) were used. Strong anti‐fusion inhibition was also observed with two other HIV strains. In addition, an increase of the anti‐HIV effect was observed when we used sera obtained by multicomponent vaccine immunization. The same kind of inhibition was also observed in p24 assay systems using these immunized antisera. Activation of IL‐2 production in lymphocytes was observed in p24 assay systems using these immunized antisera. Activation of IL‐2 production in lymphocytes was observed in mice immunized with this vaccine. These results suggest that immunization with macromolecular peptide complexes can result in strong immunogenicity towards HIV‐1.