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Identity elements of Escherichia coli tRNA Ala
Author(s) -
Tamura Koji,
Asahara Haruichi,
Himeno Hyouta,
Hasegawa Tsunemi,
Shimizu Mikio
Publication year - 1991
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.300040404
Subject(s) - transfer rna , alanine , escherichia coli , valine , biochemistry , chemistry , biology , amino acid , rna , gene
Studies using the T7 transcription system revealed that the discriminator base A 73 and the G 20 in the variable pocket play important roles in the Escherichia coli alanine tRNA identity. The C 60 in the T‐loop, which is unique to alanine tRNA, was not found to be crucial for alanine identity. Anticodon replacement into the valine anticodon UAC did not decrease alanine charging activity, and no alanine charging activity was detected in the mutant valine tRNA possessing the alanine anticodon UGC.