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New developments in affinity chromatography
Author(s) -
Lowe Christopher R.,
Burton Steven J.,
Burton Nicolas,
Stewart David J.,
Purvis Duncan R.,
Pitfield Ian,
Eapen Saji
Publication year - 1990
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.300030305
Subject(s) - affinity chromatography , adsorption , chemistry , chromatography , selectivity , yield (engineering) , combinatorial chemistry , materials science , organic chemistry , enzyme , metallurgy , catalysis
The design, synthesis and chromatographic operation of a new range of stable and selective immobilized dye affinity adsorbents for potential application in the purification of pharmaceutical proteins is described. Computer aided molecular design has been exploited to design novel dye ligands which show a predictable selectivity for the target protein and which, when coupled to stable perfluoropolymer supports, yield high capacity, low leakage adsorbents for affinity chromatography. It is anticipated that these new materials will withstand the rigorous conditions required for sanitization and cleaning in situ of industrial scale processes.