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Definition of the phage G13 receptor as structural domains of trisaccharides in Salmonella and Escherichia coli core oligosaccharides
Author(s) -
Wollin Ralfh,
Bruse Gert W.,
Jansson PerErik,
Lindberg Alf A.
Publication year - 1989
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.300020106
Subject(s) - trisaccharide , chemistry , escherichia coli , heptose , stereochemistry , oligosaccharide , receptor , biochemistry , galactose , carbohydrate conformation , nuclear magnetic resonance spectroscopy , gene , mutant
The interaction between phage G13 and different bacterial and synthetic oligosaccharides has been studied using equilibrium dialysis inhibition. The results, and conformational analysis of the oligosaccharides, make us conclude that the phage G13 carbohydrate receptor is a conformational domain involving three sugar residues. The following trisaccharide elements contain in domain: α‐ D ‐Gal p ‐(1→3)‐[α‐ D ‐Gal p ‐(1→6)]‐α‐ D ‐Glc p , α‐ D ‐Man p ‐(1→3)‐[α‐ D ‐Man p ‐(1→6)]‐α‐ D ‐Man p , and α‐ D ‐Glc p ‐(1→3)‐[ L ‐ gly ‐α‐ D ‐ man ‐Hep p ‐(1→7)]‐ L ‐ gly ‐α‐ D ‐ man ‐Hep p . Thus two structures, either a hexose substituted with α‐ D ‐glycopyranosyl groups in the 3‐ and 6‐positions, or a heptose substituted with such groups in the 3‐ and 7‐positions are functional G13 binding sites. Such domains are present in several cores of lipopolysaccharides from Salmonella and Escherichia coli species. Some cores, e.g. those from S. typhimurium chemotypes Ra, Rb 1 and Rb 2 , contain two such domains. The identification of two G13 receptor domains within different core saccharides could explain the broad host range this phage.