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Studies on a possible molecular basis for the structure of mitochondrial cristae
Author(s) -
Sumegi Balazs,
Freeman Dale A.,
Inman Lindsey,
Srere Paul A.
Publication year - 1988
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.300010105
Subject(s) - mitochondrion , cytochrome c oxidase , chemistry , chloramphenicol , electron transport chain , microbiology and biotechnology , inner mitochondrial membrane , oxidase test , biochemistry , biology , biophysics , enzyme , antibiotics
We have investigated a possible molecular basis for mitochondrial cristae formation. Proteoliposomes containing electron transport proteins, cytochrome oxidase, or complex III in their proper orientation bind to pig heart mitoplasts but not pig heart mitochondria. Using Leydig tumor cells, we have confirmed earlier reports that chloramphenicol causes a diminution in cristae content and a change in its characteristic lamellar form. We show that the proteoliposomes containing cytochrome oxidase or complex III in the proper orientation bind to mitoplasts from Leydig tumor cells but do not bind as well to mitoplasts from chloramphenicol‐treated Leydig tumor cells. These experiments provide a possible mechanism to explain cristae formation.