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Investigation of polyacrylamide hydrogel‐based molecularly imprinted polymers using protein gel electrophoresis
Author(s) -
EL Sharif Hazim F.,
Giosia Flavio,
Reddy Subrayal M.
Publication year - 2022
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2942
Subject(s) - molecularly imprinted polymer , molecular imprinting , polyacrylamide , self healing hydrogels , gel electrophoresis of proteins , chemistry , polyacrylamide gel electrophoresis , chromatography , polymer chemistry , biochemistry , selectivity , enzyme , catalysis
In conjunction with polyacrylamide gel electrophoresis (PAGE), molecular imprinting methods have been applied to produce a multilayer mini‐slab in order to evaluate how selectively and specifically a hydrogel‐based molecularly imprinted polymer (MIP) binds bovine haemoglobin (BHb, ~64.5 kDa). A three‐layer mini‐slab comprising an upper and lower layer and a MIP, or a non‐imprinted control polymer dispersion middle layer has been investigated. The discriminating MIP layer, also based on polyacrylamide, was able to specifically bind BHb molecules in preference to a protein similar in molecular weight such as bovine serum albumin (BSA, ~66 kDa). Protein staining allowed us to visualise the protein retention strength of the MIP layer under the influence of an electric field. This method could be applied to other proteins with implications in effective protein capture, disease diagnostics, and protein analysis.