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Vatairea guianensis lectin stimulates changes in gene expression and release of TNF ‐α from rat peritoneal macrophages via glycoconjugate binding
Author(s) -
Marques Gabriela Fernandes Oliveira,
Pires Alana Freitas,
Osterne Vinicius Jose Silva,
PintoJunior Vanir Reis,
Silva Ivanice Bezerra,
Martins Maria Gleiciane Queiroz,
Oliveira Messias Vital,
Gomes Angelica Maciel,
Souza Luiz Augusto Gomes,
Pavão Mauro Sérgio Gonçalves,
Cavada Benildo Sousa,
Assreuy Ana Maria Sampaio,
Nascimento Kyria Santiago
Publication year - 2021
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2922
Subject(s) - in vivo , tumor necrosis factor alpha , microbiology and biotechnology , tlr4 , lectin , receptor , in vitro , gene expression , chemistry , biology , immunology , gene , biochemistry
Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll‐like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose‐dependent gene expression and release of TNF‐α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF‐α dosage (mean ± SEM ( n = 6), analysis of variance, and Bonferroni's test ( P < .05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin‐induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF‐α gene expression (RT‐PCR) and release from peritoneal macrophages. Thus, upon lectin‐glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF‐α and TLR and/or TNFR receptor pathways.